Composite

Part:BBa_K5241009:Design

Designed by: yingmei tang   Group: iGEM24_ECIB-PKU   (2024-09-25)


GAL1-Tyrosinase(BM)-GAL1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3371
    Illegal XbaI site found at 1769
    Illegal SpeI site found at 1073
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3371
    Illegal SpeI site found at 1073
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3371
    Illegal BglII site found at 2217
    Illegal BamHI site found at 3117
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3371
    Illegal XbaI site found at 1769
    Illegal SpeI site found at 1073
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3371
    Illegal XbaI site found at 1769
    Illegal SpeI site found at 1073
    Illegal NgoMIV site found at 10303
    Illegal AgeI site found at 382
    Illegal AgeI site found at 1844
    Illegal AgeI site found at 2604
    Illegal AgeI site found at 9917
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5245
    Illegal SapI site found at 4162


Design Notes

none


Source

Priestia megaterium or Bacillus megaterium

References